Beschreibung von: A Novel Taylor-Couette Bioreactor for Mammalian Cell Cultivation

In the pharmaceutical industry mammalian cell lines
are the primary expression system in the production
of therapeutic recombinant proteins. Despite its
diverse drawbacks, the stirred tank reactor serves
here as major workhorse for most applications. To
overcome the limitations faced, improved bioreactors
are needed to provide a more favorable in vitro
environment to mimic in vivo conditions. In order to
establish a suitable culture system, the cell s
responsiveness to environmental stress is evaluated
to identify and anticipate the impact of this
process parameter during cell cultivation. Based on
these studies, a novel bioreactor featuring good
mass transfer characteristics at low shear values
both in maximal magnitude as well as distribution is
constructed. Its unique cultivation microenvironment
enhances cell growth and protein production and
makes it superior to conventional stirred tank
reactors. Especially nowadays in the environment of
a fast growing and competitive market, governmental
price control and rising quality requirements, the
proposed bioreactor provides a suitable tool for
biopharmaceutical research, development, and
production.